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I have an incoming project to which they would like us to perform metagenomic library prep and sequencing. They intend to give us gDNA environmental samples...

I am screening a library of 10-15k variants through cell-based assays. After the selection, I perform the NGS sample prep using NEB ultra II kits. When...

During library preparation, DNA or RNA targets are processed and converted into the appropriate format for a sequencing run. Library preparation workflows can differ greatly between kits and the needs of the experiment. Each library prep kit contains different components necessary to prepare the target nucleic acid for sequencing. The constant development of sequencing techniques has led to a growing number of library prep options, and the process of identifying the...

SPRI beads and magnetic beads are well used in molecular labs for nucleic acids purification because they are fast, simple and efficient. The magnetic...

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Genome, epigenome, transcriptome, and metagenome sequencing are all constantly evolving and finding new applications for...

Good Morning All,

I am trying to sequence a few hard-to-ID Enterobacteria using the Oxford Nanopore Rapid Barcoding Kit (SQK-RBK004) on the...

Hey all,

I'm looking to sequence a handful of pure bacterial isolates for WGS and de novo assembly. Our group has decided to go with a combined...

Hello to you all,

I want to perform DNA seq and I am having issues in the step of ligating the adaptors to the sequences.

...

Hi,
I've prepared ChIP-Seq libraries using NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335) along with the NEBNext Ultra II DNA Library...

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I am interested in performing polysome profiling with downstream application for RNAseq of monosome and polysome-associated RNAs....

I have a Hi-C library with P5 and P7 adapters. I would like to enrich for fragments containing a locus-of-interest by capture and extension with a biotinylated...

Am 8. Juli um 14:00 Uhr findet ein Illumina Webinar zum Thema RNA Library Prep statt.

Es werden folgende Themen behandelt:
- Einführung...

Hi all,

I've been having this issue that has been plaguing my PhD. Some of my sequencing runs come back with terrible uneven read coverage....

How Fast?
1-2 hours protocols

How Simple?
10 min of hands-on time

What you get:

• Fast protocol
  - 1-1.5

...