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Repeatscout output
Hello, I have mygenome.fa and I used repeatscout to produce mygenome_repeats and then I filtered it. Now I have mygenome_repeats_filtered.fa. what is...
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To mask or not to mask?
Hi all,
I read several threads here and in other bio-forums about masking genome regions. There are different opinions, from don't mask...
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question about cufflinks masking
Hello all,
I recently created a merged.gtf file using cuffmerge. I want to mask all the known transcripts in this file when using cufflinks...
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When is repeat masking necessary?
Hi all
I am planning to work on some comparative genomics where I look at secondary metabolite biosynthetic genes in multiple species of...
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indexing the nt database with makembindex to improve blastn (task megablast) runtime
I am trying to dust mask the NT database and then to index it using makembindex. However, when I run megablast on the indexed and dust masked nt database...
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casava 1.8.2 adapter masking new option
Hello,
I am starting to work with casava 1.8.2 for demultiplexing data with samples with dual and single index in the same paired-end Illumina...
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Mapped onto pseudogenes?
I have a problem in TopHat-Cufflinks pipeline. I want to get splicing information of a gene family, but the annotation is bad so far, that is, nothing...