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  • asapp
    Junior Member
    • Jun 2015
    • 8

    question about cufflinks masking

    Hello all,

    I recently created a merged.gtf file using cuffmerge. I want to mask all the known transcripts in this file when using cufflinks to search for novel transcripts at a particular locus (using the --mask-file <mask.gtf> option in cufflinks).

    Here’s my question: can anyone tell me if --mask-file masks the entire transcript from assembly (i.e. from beginning to end irrespective of exon-intron structure) or just the identified exons, leaving the introns unmasked?

    Thanks in advance for you help.
    -Anna

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