Hi,
I know this is an obsolete technology, but I came across some data produced on the 454 instrument (archived in SRA/ENA) which I'd like to use...
17 results in 0.0029 seconds.
-
454 GS FLX Titanium 'paired end' protocol
-
metasim
Hello
I am a research student and I have a seminar on the introduction of METASIM software ... Unfortunately, I do not have a good background about... -
Three reads with the same name in the BAM file
Hi all,
I am dealing with the paired-end BAM file, and come up with many warnings like this:
...Code:WARNING: Could not find pair
-
Paired end in Ion Torrent
Hi you all!
I need your help... I'm trying to search for a translocation using ion torrent sequencing (i know the genes involved in the... -
Mate Pair QC
I've been using a combo of fastqc and cutadapt to qc some mate pair libraries we have.
This is a Truseq LT matepair library
... -
NxSeq Long Mate Pair Technology for up to 20 kb mate pair libraries
Hello SEQanswers users,
Lucigen would like to announce the availability of our new NxSeq Long Mate Pair library kits and services. The product... -
How to pair mates after de-barcoding by fastx BarCode splitter?
I have been having some issues doing a paired end analysis simply due to the way I am processing things.
I currently use custom illumina... -
genome assembly with only mate pair reads
Hi,
I am mostly comfortable with DNA resequencing, mRNAseq, ChIPseq, etc. data. And always feel difficult handling de novo assembly works.... -
Mate pair sequencing - quality, duplication, throughput
We recently got Illumina mate pair sequencing data for a human sample. I am trying to do some some Quality Check to see how good the data is.
... -
Scaffolding despite multiple links in SSPACE
I have denovo assembled a 1.8 Mbp genome with a PE 100 bp 360 insert library using abyss resulting in 69 contigs. After that I scaffolded these contigs... -
mate pair insert size variation and de novo assembly
Hi All
One of my lab bench colleagues is working on a constructing an Illumina mate pair library intended for use in a target capture procedure... -
Looking for a pipeline - SV detection mit mate pair
Hey guys,
I am trying to set up asimulation to estimate what mate pair coverage I need to detect a big deletion in my cthromosom. The steps... -
Rescuing Orphaned reads with local alignment within a radius of mapped mate
So we have a dataset of variable insert library as we are sequencing the 5' and 3' end of transcripts. As a result the distance between the mates( <---... -
Split mate pair 454 for newbler
Hi,
I have a bunch of 454 3kb mate pair files in SFF format which I'd like to use to get a Newbler assembly. However, I want Newbler to... -
anyone with expertise on cDNA mate-pair library?
Would like to know, if anyone has made a mate-pair library (preferably for Illumina) using RNA as the starting material. I found a PET protocol (454)...
