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  • Rescuing Orphaned reads with local alignment within a radius of mapped mate

    So we have a dataset of variable insert library as we are sequencing the 5' and 3' end of transcripts. As a result the distance between the mates( <--- --->) is dependent on the length of transcript. To map the reads initially I am first using Mosaik which i belv does a better job with variable insert mate pair data.

    After mapping we still see 40% orphaned reads where one read maps and the other doesn't. Is there a way that I can do a local re-alignment for these orphaned reads and attempt to map the mate within a given radius of the mapped mate.

    Anything already out there ?


    Thanks!
    -Abhi

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