Hello!
I am using 454 Roche Junior, I have few questions about reads of sff file.
I have splitted a SFF file into individual SFF file (according...
8 results in 0.0045 seconds.
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SFF file - primers
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Need help to prepare sample for pyrosequencing
Hi
I am quite new to Pyrosequencing and therefore have some strange question!
I am going to analyse 60 gut samples using... -
Multiplexing more than 384 samples on a MiSeq run
Does anyone know of any good methods for multiplexing more than 384 samples on a MiSeq run? The Nextera XT Index Kit allows indexing for a maximum of... -
454 Junior MIDs
Hey folks.
I'm attempting to do a 454 Junior run with 54 pooled libraries, and it seems that there are only 14 suggested MIDs for use... -
unequal read representation
Hi everybody
I am using a GS Junior to sequence a rapid library of DNA fragments which start and end with an individual specific tag. Even though... -
weird 454 reads
Hello
I have a weird 454 result produced by MID and DSN normalization.
The mRNA reads started with 'key(4bp)+MID(10bp)+cDNA library primer... -
GS FLX Mate Paired End library Construction with MID
Is there anyone who have any information about "GS FLX Mate
Paired End library construction with MID"? -
454 sequence data filtering
Hi all!
I'm working on a pipeline that deals with 454 data analysis. First step is to filter the 454 sequences, thus removing primers,...
