Does anyone know of any good methods for multiplexing more than 384 samples on a MiSeq run? The Nextera XT Index Kit allows indexing for a maximum of 384 samples. We have already purchased one of these kits, but would like to add more samples onto our run. I know the Illumina customer letter provided sequences for some of their barcodes, but not for all of them. Does anyone know if the rest of these are available elsewhere? I suppose it would be possible to order oligos identical to the Nextera barcode adaptors but with different 8 bp barcode sequences. Any thoughts?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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