275 results in 0.0041 seconds.
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MiSeq i100 vs MiSeq (OG)
Soon, we may have to replace our MiSeq (OG), if the EOL notice is too be believed. For those who have switched to the i100, or i100+, what are your thoughts... -
Miseq
Hello, I work with the Miseq from illumina, I have been looking all around for an answer but cannot find one. When you open the reagent cooler door is... -
Questions about ITS Region Amplicon Sequencing Quality Using Illumina MiSeq
Hi, all.
I'm analyzing fungal ITS amplicon data from gut samples. However, the data quality isn't very good—there's a noticeable drop in... -
Qiagen CLC Workbench QIAseqPRO targeted DNA panels - commercial vs own customization of templates
Hi all,
I am currently setting up two QIAseq PRO targeted DNA panels for the analysis of DNA from human FFPE samples in my lab. We are using... -
Comparison between Iongene S5 Plus and Nextseq 550 DX
Hi, at our lab we are going to install a NGS facility mainly for diagnostic and pharmacogenomics purpose. I am confused between which system should we... -
MiSeq Cycle 1 Possible Clustering Failure
We have run the same library on two different machines using a MiSeq V2 300 cycle kit and it has failed in cycle 1 both times. We are following the same... -
Non-responsive over clustering issue
Hello,
I'm sequencing 16S libraries (prepared and sequenced according to the EMP protocol) on MiSeq using a V2 kit. We used to load 12pM... -
Low quality for index1 (i7) but not for read2
Hi,
I am sequencing libraries on MiSeq (V2 and V3) and facing constant problems with my i7 reads. At the end of my read1, the quality drops and... -
Specify Bowtie2 to align a mate pair only to the same RefSeq
Hey everyone,
I am doing exome sequencing of multiple genes, of which some share great homology.
Some of the read pairs don't share... -
Multi-marker metabarcoding: how much length difference will give marker bias?
Hello SEQanswers community! I want to sequence bacterial, fungal, plant and arthropod libraries on one MiSeq run from the same faecal samples. I know... -
Pooling of amplicon libraries for MiSeq
Hi folks,
I have limited experience in pooling libraries but now I am trying to pool (fungal ITS1) amplicon libraries for MiSeq V3 600 cycles.... -
quality score issues
Hi all,
i am wondering what are possible causes of low Q30 scores, apart from overclustering?
I am working with amplicon... -
Why do some index sequences have an incorrect base?
Hello everyone,
I prepared a custom library using dual index TruSeq adapters for a Miseq run with a sample plexity of 3. This is the first... -
PhiX % for amplicon MethylSeq
What % of PhiX spike would you do for an amplicon sequencing panel made with Bisulfite-converted DNA? I have been told previously to use 25%. I want... -
errors at the beginning of hybrid assembly using MaSuRCA-4.0.3
Hi
im trying to make hybrid assembly for Ecoli using MiSeq and Nanopore data in Masurca. currently i m getting two errors marked in Bold...
