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Upcoming online UC Davis Bioinformatics Core workshops
Join us in July and August to explore genome assembly, mRNA-Seq analysis, and Single-Cell analysis! We’re excited to follow up our three recent successful... -
CAPPED-mRNA sequencing
Hi,
Has anyone tried to enrich for capped-mRNA (of virus)vfrom total mRNA?
if yes, what kind of method did you use?
I know... -
mRNA-seq experiment: how to deal with technical replicates
Dear all,
I would like to have our opinion on how to deal with technical replicates in a mRNA-seq experiment for DGE analysis. I saw multiple... -
How to calculate standard deviation between replicate in mRNA seq data?
Dear all,
I am working with RNA-seq data and I am new to this kind of data. I have mrna seq data with 8 conditions and each having three replicates.... -
Validation algorithm.
Hi.
I work in the development of algorithm for work with differential expression with data from mRNA-Seq.
Now I need validate this... -
Lexogen announces the "Win your SENSE Study" award
Lexogen’s new SENSE mRNA-Seq kit gives researchers the power to analyze antisense transcription like never before. By eliminating the causes of false-positive... -
Unusually high FPKM for cufflinks
Hi,
I have been working with Cufflinks v. 1.1.0 with mRNA-Seq data from some of the old (2008) runs of Illumina with 36bp reads. The only...Last edited by flobpf; 02-13-2012, 01:21 PM. -
no index reads
Hello HiSeq2000 users,
we just finished a multiplexed mRNA-seq run on the HiSeq2000 with puzzling results, maybe somebody encountered this... -
High duplication levels in FASTQC
Hi,
I was using FASTQC to QC my directional mRNA-Seq data obtained from suspension culture cells. I have about 30 million reads.
... -
Bioanalyzer Peaks
Using the illumina mRNA Seq kits I created 8 libraries last week; they are completed through pcr enrichment and ready to run on the GAs. For the most... -
BWA parameters for mRNA-seq aligning against mRNA refseq
Hello
I am sure similar questions were posted earlier but I could not find the definitive answer.
So the question is what... -
mRNA fragmentation for SPRIworks
We are making ds-cDNA for automated mRNA-seq library preparation using the SPRIworks robot. After the poly-A selection, our protocol calls for fragmentation...Last edited by jowenMIT; 01-27-2011, 02:23 PM. -
Starting out with mRNA-seq analysis
So I'm a newbie to this so have a few questions on what programs to use (just to get me started) that are good for the beginner, I've had a good look... -
Blat vs bowtie/tophat on Rnaseq data
Dear all,
This is my first thread here, so hello everyone!
I am new in the field, so I hope my questions are not too trivial and... -
Quantifying trascript levels using RNA-seq
Hi guys,
I thought of asking this:
1. Can we get sequencing data of a certain mrna from RNA-seq, say from heart and muscle,...
