Hi,
I'm very new to sequencing so apologies if my question is naive.
I am trying to sequence whole human genome samples...
26 results in 0.0031 seconds.
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Single index adapters for low-plex
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Multiplexing BluePippin size selection
First post, but a longtime lurker!
Currently doing Illumina library preps for MiSeq sequencing, and even though we have mostly used TruSeq... -
Good index sequences for unique dual indexing?
After the index-hopping scandal (see Illumina's white paper) it sounds like the field is moving in the direction of unique dual indexing, i.e. dual indexing... -
New ONT System: GridION X5
https://nanoporetech.com/products/gridion
ONT has two separate purchase models: CapEx ($125k upfront + $300 per flow cell) and OpEx ($0 upfront...Last edited by gringer; 03-15-2017, 01:51 AM. -
Custom multiplex sequencing barcodes / indexes (8-mer)
Hi all,
I have synthesised a custom set of Illumina adaptors that use an 8-mer index to allow 384-plex multiplexing. I got these index... -
MiSeq; which ITS ?
Which are the best ITS primers to use for sequencing of environmental samples? Illumina suggests primers for 16S in the protocol but there aren't any... -
Recommended output or reads for 16s metagenomic
I am planning to use Ion 16S Metagenomics Kit (7 hypervariable region) and Ion PGM 400bp for my 16s metagenomic project. Can anyone suggesting how many... -
Ugly gels after switching to 384w plate from 96
Hello,
My lab use's custom primer sets for HLA genotyping using the Illumina MiSEQ. We have optimized our multiplex primer sets for initial amplification... -
Specifying read group info for multiple samples per lane in SAM/BAM files (for GATK)
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Feedback on RAD-seq strategy
Hi,
I'm designing an experiment for RAD-seq and I was wondering if I could get some feedback about my experimental design.
I... -
Any experience with amplicon sequencing of MHC loci on Illumina?
We are trying to decide between MiSeq and 454 FLX for a MHC genotyping project in one bird species (MHC class II B exon2, 180bp). After running a preliminary... -
combine individuals and pool in the same multiplex?
I want to perform targeted resequencing of genes for SNP discovery and CNV analysis.
I'm sequencing genes in cattle, mostly working within one... -
Add indexes to standard PE adapters?
I am interested in modifying my standard PE adapters with indexes during the final amplification step. Has anyone tried this? Have you heard/read anything... -
ChIP-Seq multiplexing
Hi all,
I have prepared a ChIP-Seq library with Illumina's ChIP-Seq DNA sample prep kit (IP-102-1001). It is in pretty good shape so I... -
ultra-high multiplex pcr -custom method?
Hi,
I would like to use an ultra-high multiplex PCR method to create libraries for the Illumina Miseq.
My aim is to create...
