Hi,
I would like to use an ultra-high multiplex PCR method to create libraries for the Illumina Miseq.
My aim is to create a panel of 500 amplicons, that can be amplified in a single reaction, but I would like to avoid using a proprietory kit such as the IonAmpliSeq.
Does anyone have a custom ultra-high multiplex PCR protocol that they could share with me? The panel will be for routine work so I want to avoid using sequence capture methods.
Kind regards,
Oliver
I would like to use an ultra-high multiplex PCR method to create libraries for the Illumina Miseq.
My aim is to create a panel of 500 amplicons, that can be amplified in a single reaction, but I would like to avoid using a proprietory kit such as the IonAmpliSeq.
Does anyone have a custom ultra-high multiplex PCR protocol that they could share with me? The panel will be for routine work so I want to avoid using sequence capture methods.
Kind regards,
Oliver
Comment