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Why is my DNA concentration and absorbance ratios fluctuating?
I have done DNA extraction on human saliva using Promega ReliaPrep™ Blood gDNA Miniprep System that have been expired for more than five years now.... -
TRIzol contamination in low yield RNA extraction.
SCENARIO:
- Goal: RNA-seq.
- Number of samples (already extracted): 200.
- Size of individual sample: VERY small (single zebrafish... -
Qubit vs Nanodrop discrepancies??
Hi all,
What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?
Our core facility has generated... -
RNA quality for library generation: Low 260/230 ratio
Hello,
I am having issues isolating quality RNA for gene expression profiling.
I isolate total RNA from samples stored... -
Library invisible on gel, despite Nanodrop results. Gel extraction problem?
I'm having some difficulty with my very first Illumina library prep, and was hoping someone might point out any obvious errors I'm committing in my ignorance.... -
UV spectra of some common contaminants of DNA or RNA solutions.
Spectrophotometers, such as the Nanodrop, can quickly produce a UV spectrum from a 2ul drop of solution without a cuvette. Gone are the days when one...
Last edited by pmiguel; 05-27-2025, 09:18 AM.
