Qubit vs Nanodrop discrepancies??

amylee86

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Join Date: Feb 2013

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#1

Qubit vs Nanodrop discrepancies??

02-15-2013, 12:04 PM

Hi all,

What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?

Our core facility has generated Qubit values for my cDNA sampels that are consistently 1.3- to 2.3-fold higher than the Nanodrop values that I generated. I've read a lot of reports claiming that Nanodrop consistently overestimates concentration, so I'm a little puzzled by these Qubit results.

Any help is greatly appreciated!

Thanks in advance,
Amy
Tags: cdna, dsdna, nanodrop, quantification, qubit
LB2301

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Join Date: Jul 2013

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#2

07-10-2013, 07:50 AM

I have this same problem with my samples!! you managed to solve your doubts? please if your answer is positive, I discuss how? I have this same problem and I think that may be the differences in temperature between rooms!!

thank you
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Qubit vs Nanodrop discrepancies??

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