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  • amylee86
    Junior Member
    • Feb 2013
    • 2
    #1

    Qubit vs Nanodrop discrepancies??

    Hi all,

    What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?

    Our core facility has generated Qubit values for my cDNA sampels that are consistently 1.3- to 2.3-fold higher than the Nanodrop values that I generated. I've read a lot of reports claiming that Nanodrop consistently overestimates concentration, so I'm a little puzzled by these Qubit results.

    Any help is greatly appreciated!

    Thanks in advance,
    Amy
    Tags: cdna, dsdna, nanodrop, quantification, qubit
    • LB2301
      Junior Member
      • Jul 2013
      • 1
      #2
      I have this same problem with my samples!! you managed to solve your doubts? please if your answer is positive, I discuss how? I have this same problem and I think that may be the differences in temperature between rooms!!

      thank you

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