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How to scale up library prep for large quantity of input DNA?
I am screening a library of 10-15k variants through cell-based assays. After the selection, I perform the NGS sample prep using NEB ultra II kits. When... -
Total RNAseq library - artifact levels up to 95% with clear pattern on 96 well plate
I have (very complex) artifact levels up to 95% in my total RNAseq data.
Some facts:
- NEB Ultra 2 Library prep
- 96 samples per plate,... -
Mixing pooled libraries at the same time
Hello everyone,
I am a new member of SEQanswers. I am planning to run MiniSeq system for the first time. Unluckily, I have a big problem....
