Hi all,
We ordered cassettes for our Blue Pippin back in March and are still getting the run around from the company (https://sagescie...
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Sage Science Blue Pippin Supplies unavailable
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Genomics Core Manager - Fort Lauderdale, FL
Location: Fort Lauderdale, FL
Nova Southeastern University’s Translational Research and Economic Development Department is seeking a... -
Illumina NextSeq shorter fragment size than expected
Hi,
We were preparing library for NextSeq whole exome sequencing. Our starting concentration before library preparation for three samples... -
Can I see the detailed information of off-target reads of sequencing on NextSeq 500?
I would like to see the off-target read details of the run on NExtSeq 500 with TruSeq Rapid Exome kit?
I know that Ihave lots of reads... -
nextseq 500 - service discontinued?
I hear rumors that service on the Nextseq 500 has been discontinued. Has anyone had issues getting service contract, parts or reagents on their Nextseq... -
Low Exome Coverage with Truseq DNA Exome and NextSeq 500 workflow?
Dear All,
I studied a whole exome with Truseq DNA Exome (2x75 and suitable High output kit) on NextSeq 500.. AFter sequencing I got around... -
calctruequality in bbmap
I'm trying to recalibrate Q scores of a NextSeq run using MiSeq contigs assembled with Tadpole. The commands I used to map the reads to the reference... -
Weird overrepresented K-mers in FastQC
Hi! We have recently done the first run on a new NextSeq 500 machine which produced reads with weird FastQC results (attaching below).
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NextSeq having problem sequencing amplicons with stretch of C
We seems to have problem sequencing several different amplicons with a stretch of C on the NextSeq using v2 chemistry 300-cycle kits. This happen to 2... -
Optimizing on NextSeq
Howdy,
I work for a clinical genomics company, we have a NextSeq 500 and have been trying to squeeze the most reads per sample out of it.... -
Failed NextSeq 500 v2 runs w/ custom sequencing primer
Hi,
Any tips regarding the use of a custom primer on the NextSeq would be appreciated.
We are trying to run a multiplexed set...Last edited by kakseq; 06-01-2015, 10:05 AM. -
Nextseq500 N's from position 25
Hi,
I've been seeing a lot of Nextseq500 runs lately and I found a very disturbing phenomenon. In a lot of the runs I start seeing a lot of N's... -
NextSeq Data
We recently acquired a NextSeq machine and are not very impressed with the data. I've uploaded a spreadsheet containing some of the statistics here:... -
How much DNA contamination "allowed"?
Hi,
We're going to do a RNA-SEQ experiment (with Nextseq 500) with bacterial RNA for differential gene expression. We have cells lysed... -
100 Gb Data/Day – Nextseq 500 Sequencing Services Now Available on Genohub
We're pleased to announce you can order NextSeq 500 sequencing services on Genohub.com today: https://genohub.com/shop-by-next-gen-sequencing-technol...
