100 Gb Data/Day – Nextseq 500 Sequencing Services Now Available on Genohub

[Genohub]

We're pleased to announce you can order NextSeq 500 sequencing services on Genohub.com today: https://genohub.com/shop-by-next-gen...48c30d3f93d505

We expect the highest interest to be centered around targeted sequencing (exome or custom regions) and fast RNA profiling. For exome studies, you can run between 1-12 samples in a single run and get back 4 Gb at 2 x75 or 5 Gb at a 2×100 read length. If you’re interested in RNA profiling at 10M reads per sample, you can multiplex between 12-36 samples together in a single run. A 1×75 cycle run takes as few as 11 hours to complete and 2×150 runs take ~29 hours.

For more details: http://blog.genohub.com/100-gb-of-da...le-on-genohub/

[SNPsaurus]

Any idea if the NextSeq is more MiSeq or HiSeq-like in its tolerance for low-diversity reads (16S, small number of amplicons, etc)?

[Genohub]

The NextSeq is less tolerant than HiSeq for low-diversity reads. NextSeq uses a two channel SBS sequencing process. It's easier for current software versions to differentiate cross-talk matrix with 4 dyes. MiSeq is still the way to go for amplicon-seq, 16S. We've written a few words about it here:

https://genohub.com/ngs-instrument-guide/#illumina

- Genohub

[SNPsaurus]

Good thoughts.