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Onso from Pacific Biosciences: Latest Sequencing Technologies—Part 4
- Published: 12-22-2022, 01:20 PM
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in ArticlesOnso from Pacific Biosciences: Latest Sequencing Technologies—Part 4
Onso was the second sequencer Pacific Biosciences (PacBio) revealed during this year’s American Society of Human Genetics meeting (read about the first sequencer here). This benchtop sequencer uses sequencing by binding (SBB), a method unlike any other instrument on the market. SBB incorporates native nucleotides, has reduced molecular scarring, and reportedly results in significantly higher accuracy base calls than traditional short-read sequencers. The following sections highlight the important...
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Revio from Pacific Biosciences: Latest Sequencing Technologies—Part 3
- Published: 12-22-2022, 01:19 PM
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in ArticlesRevio from Pacific Biosciences: Latest Sequencing Technologies—Part 3
Pacific Biosciences (PacBio) revealed two new sequencers during this year’s American Society of Human Genetics meeting. The first instrument, Revio, which will be covered in this article, is a significant upgrade from their previous Sequel II device. It has lower costs, shorter run times, more SMRT Cells and zero-mode waveguides (ZMW), and significantly higher output compared to PacBio’s other sequencers.
Read type and length
As a part of PacBio’s long-read sequencing systems,... -
pacbio sequel system
how much do core facilities charge to run the instrument, not per sample or SMRTcell but just to recover the cost of running the device? (electricity,...
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Combining PacBio assembly with 10X assembly?
Dear all,
Currently we created an assembly of a highly repetitive plant genome with
60x coverage of Pacbio reads (assembler HGAP4).
...
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Can I use velvet to assemble SMRT reads?
I don't know whether it's possible to use velvet to assemble SMRT reads. Also I want to know how to know the read coverage of SMRT. Is that a function...
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Postdoc or Scientist, Third-gen Sequencing and Integrative Epigenomics (NYC)
In Brief: Epigenomics, PacBio, Oxford Nanopore, RNA Sequencing, Systems/Network biology of infectious diseases and genetic disorders.
Job...
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FALCON assembler without SGE?
Has anyone had any success getting FALCON to work on a single node without SGE installed?
-Jason
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FinisherSC
I want to introduce the following tool for upgrading pacbio assemblies.
The link of the tool is http://kakitone.github.io/finishingTo...
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PacBio RS II Library Prep Input
Hi all,
I'm looking to sequence low quantities of PCR amplicon using the PacBio RS II system. I am trying to detect single nucleotide variants...
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Pacific Bio releases some details on SMRT Sequencer read lengths, library prep
Our friends over at In Sequence posted a very interesting article containing details from Pacific Biosciences regarding their much anticipated third-gen...