Hi all,
I have been working on two genes which I want to sequence later on. Initially I tried amplifying them using Duplex PCR protocol....
47 results in 0.0024 seconds.
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Duplex PCR not working
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Can primers for PCR be duplicated?
Complete beginner question here, don't laugh: If I have some primers that have been synthesized, and I am close to running out of them, is there any way... -
Non-specific bands when using Illumina adapters?
Hi all,
I am new to the Illumina system. When I did the PCR and ran the gel using normal sets of primers. Everything is nice and clean. However,... -
Thermal cycler with variable temperature lid?
Does this machine exist:, for example, a machine that can change the lid temperature during the course of the reactions? Something tells me that having... -
Tools for cleaning PCR hood?
I have a really hard time reaching the back walls of my PCR hood to clean it. Does anyone know of any specific tools you can use to make it easier to... -
Low input of primers for pre optimized PCR step
Hello,
I have created a situation where I only have enough reagents to add approximately 1/5 of the primers recommended (pre optimized by Illumina)... -
Inexpensive, high throughput DNA methylation quantification
I have a probe list (varies between 200 to 7,000 probes) from the Illumina 450K Beadchip that I'd like to quantify as cheaply as possible. Needs to be... -
ddRAD: Performing PCR BEFORE Size-Selection?
Greetings!
A quick search yielded a thread asking a similar question, but it went unanswered.
Up-front, my question is:... -
Library PCR cycle optimisation
Hi there,
This is probably a very naive question, but how can I test the number of cycles required to do library enrichment, if I have... -
Demultiplexing PacBio sequences
Hi all,
I have the following problem:
I have a dataset containing circurlarized and linear DNA. It was amplified with two pairs of primers... -
Best approach for targeted PCR resequencing on MiSeq from RNA
Hi everyone,
I need to amplify selected regions from my RNA samples and resequence with MiSeq.
I am unclear to what is the... -
Automated primer walking
Hi all,
I'm trying to generate a list of oligonucleotide primers for Sanger sequencing, such that for an arbitrarily large DNA sequence I would... -
TruSight One - no library
Hi everyone,
I'm running the TruSight One panel, and the samples look great on Qubit and Bioanalyzer after the 1st PCR. However, at the end of... -
Counting Reads in BAM file per genomic position
Hello,
I am looking for a tool or script that counts the number of reads in a bam file by position, and takes into account only successfully...Last edited by C9r1y; 11-24-2015, 02:31 PM. -
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