6 results in 0.0054 seconds.
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MiSeq Cycle 1 Possible Clustering Failure
We have run the same library on two different machines using a MiSeq V2 300 cycle kit and it has failed in cycle 1 both times. We are following the same... -
% PhiX for Illumina sequencing protocols and SAV interpretation?
Hello,
I am a bit confused on how to calculate the volume of PhiX to add to the sample prior to sequencing on the Illumina platform. Based... -
PhiX % for amplicon MethylSeq
What % of PhiX spike would you do for an amplicon sequencing panel made with Bisulfite-converted DNA? I have been told previously to use 25%. I want... -
cluster density problem in TruSeq Small RNA library sequencing
Dear all
I’ve sent a 8-indecies pooled sample for TruSeq Small RNA library illumina sequencing. Mention that I didn’t use kit and I used CATS... -
16s MiSeq Illumina library concentration and PhiX
Hi all,
I have 2 small questions concerning the 16s sequencing protocol for MiSEq:
1) Some samples have very low concentration... -
PhiX control lane in Hiseq2500
Hello,
I would like to know if you are running phiX in a separate lane to calculate a matrix in HiSeq2500 runs.
And if...
