Hello,
I am having an issue with library pools rapidly degrading after pooling and size selection.
I am prepping low biomass...
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Library degrading after prep with Ovation Ultralow V2
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TruSeq DNA Single Indexes Sets: How many samples on one lane
Hi
I'm new to Illumina sequencing and would like to run a few tests on a MiSeq platform using a TruSeq DNA Nano kit.
As... -
Pooling and sequencing NexteraXT and TrueSeq PCR free libraries on a single lane
Hi everyone,
I have a question regarding the pooling of NexteraXT and TrueSeq PCR free libraries onto a single Illumina HighSeq lane. I... -
non-equimolar pooling to normalise read depth in libraries with different genome size
I'd like to sequence libraries of very different genome size on a single MiSeq run.
Eight bacterial samples with 4.5Mb genome size
... -
phiX on Bioanalyzer
In case anyone else was curious. This is Illumina's ΦX174 reference library ("PhiX Sequencing Control V3", p/n FC-110-3001, allegedly 10... -
Pooling Prior to Size-Selection
Greetings everyone,
I am a graduate student making his first foray into NGS. Briefly, I am employing a ddRAD protocol to generate my first... -
Treatment of ChIP-Seq Input
Hello all! I have placed this in the Bioinformatics forum because I believe this might be a more statistical problem, but if I'm incorrect, please don't... -
ddRAD size selection with Ampure beads
Hi there,
I am looking at using a modified ddRAD protocol in a genus of plants with a genome approximately 1000Mbp in size. I do not have... -
combine individuals and pool in the same multiplex?
I want to perform targeted resequencing of genes for SNP discovery and CNV analysis.
I'm sequencing genes in cattle, mostly working within one... -
pooling long range PCR
Hello, I have an experiment with 20 over lapping long range PCR fragments that span a single gene. The average size is 5kb (max 10, min 1.5kb) with equimolar... -
stuck in normalize and pool step TruSeq
Hello,
Well today I feel accomplished because I manage to almost finish my library prep successfully. Now the next step got me... -
Diff expression: pooling reads of replicates/treatments for de-novo assembly
I want to make pairwise comparisons of gene expression between tissue samples (same tissue, same species, different individuals) in 5 different treatments... -
Pooling for emPCR
Does anyone struggle with the SOLiD™ Library TaqMan® qPCR Module for quantification of SureSelect Exomes?
We are finding that our coverage... -
DESeq - sharing mode question, and an odd p-value distribution
I have a couple of questions about DESeq (and my particular analysis)
1) I have a data set with three controls and three treated samples.... -
Astronomical quotation
Hi everyone,
I hope you can help me, I have requested a quotation from a sequencing service company and the price is not what we expected....
