stuck in normalize and pool step TruSeq

anamar

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Join Date: Sep 2011

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stuck in normalize and pool step TruSeq

03-01-2013, 09:18 AM

Hello,

Well today I feel accomplished because I manage to almost finish my library prep successfully. Now the next step got me thinking. Normalize and pool. I run the bioanalyzer 2100 and I wanted to start making the calculations for normalizing. Is there a unique value -as in the bioanalyser results- for normalizing each library? there is a list of the peaks with its own molarity value, should I just make an average of them? should I just add them? I know the answer must be some very basic stuff, but I would really appreciate your help,

all the best
Tags: bioanalyzer, molarity, normalisation, pooling, truseq

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