Hi there!
Anyone working with Illumina Nextera DNA Flex for Enrichment?
We are experiencing some problems related to the hybridization...
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Problem with UCSC GTF files??
Hi,
I would like to ask for some opinion and advice related to the different available GTF-file sources for annotated genes.(mm10, but...
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Question: (ERR): bowtie2-align exited with value 1
Hi I have a problem when run tophat. I have recieved this massage:
Error running bowtie:
Warning: output file '-' was specified without...
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Problems with Biolinux 8
Hi everyone, I am quite new on bioinformatics and I trying to learn, I am doing this on biolinux8, but now it doesn't boot, and is asking me to upgrade...
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Problems in data analysis
Dear all,
I am doing a research among bioinformaticians, and I have prepared a short survey with questions regarding data analysis. I...
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Linux Mint: Not playing nice with bioconductor/edgeR. Why?
I am running Linux Mint (a variant of Ubuntu basically). I get the following warning messages:
...Code:> source("http://bioconductor.org/biocLite.R")
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Problems using NexteraXT with mitochondrial DNA
Hi all,
I just prepared a NexteraXT library of mitochondrial DNA for a collaborator. Although they purified the mitochondrial DNA (removed...
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Newbie at odds with large alignment
Greetings everybody,
I'm new to the world of sequencing, and I'm trying to develop a metagenomic project for my university.
We had some...
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BWA Alignment Segmentation Fault
I'm trying to align a set of 51bp paired-end Illumina reads from a human cell line cDNA library. BWA does a great job aligning my reads to the indexed...
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illumina2srf error: .params file not found
Hi all,
I'm running the illumina2srf script on my Solexa runs to generate the correspondent srf files. During the processing of every tile...
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Maq problems getting started
I have downloaded Maq & am trying to get the program to work.
When I try to run Maq I get this output
maq.pl demo ref.fasta...