Hello,
We've been having some quantitation issues recently, I'm wondering whether the Qubit fluorometers are good for processing lots...
9 results in 0.0021 seconds.
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Is Qubit good for multiple samples?
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strong 140bp peak following RNA library construction
Hello all,
I have created an illumina truseq library on bacteria by first performing ribozero rRNA depletion kit and then performing a truseq... -
RNA-Seq quantitation for human, paired end vs single end, strand specific or not?
Is there any consensus of opinion on the best RNA-Seq approach for quantitation of alternative splicing on well annotated genomes (e.g. human)? We don’t... -
Improved ChIP-Seq Quantitation
Do you need to improve the quantitation of your ChIP-Seq experiments? Molecular Indexes™ can be incorporated into your library prep, thus allowing identical... -
qPCR settings
Hello,
What threshold do you set for a qPCR run for quantifying libraries? We use KAPA Library Quantification Kits on an Eco instrument. We currently... -
Anyone used the Promega GloMax Multi Plus for quant?
Hi there,
Any one used the Promega GloMax Multi Plus (fluoro plate reader) for genomic DNA quant? Everyone seems to use the Qubit - even... -
96 well fluorometer for genomic DNA quant?
Hi there, we will soon be launching a high-throughput NGS service. We currently do all our genomic DNA quant on the Qubit (as we have had problems with... -
Quantifying trascript levels using RNA-seq
Hi guys,
I thought of asking this:
1. Can we get sequencing data of a certain mrna from RNA-seq, say from heart and muscle,... -
ChIP DNA Quantitation with Low-Range PicoGreen Standard Curve
Hello everyone,
We’ve been having problems using the PicoGreen assay to determine our final ChIP DNA samples prior to sample prep for...
