Hello,
We received the raw reads of our ddRAD library from a sequencing facility, and after demultiplexing, I noticed that there is a 10-fold...
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Normalization from different NGS runs
Hello community, I would certainly appreciate some help here. Many thanks in advance.
I have been looking around about this subject and everything...
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Counting Reads in BAM file per genomic position
Hello,
I am looking for a tool or script that counts the number of reads in a bam file by position, and takes into account only successfully...Last edited by C9r1y; 11-24-2015, 02:31 PM.
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Tools that report read count AND read names that map to genomic features.
htseq-count and coverageBed are excellent tools for reporting just how many reads map to each genomic features.
How ever to my knowledge...
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Data Analysis Suggestion for a Newbie?
Hi all, I am a newbie in Bioinformatics. I've got data for paired-end DNA sequencing on Illumina platform and I am wondering if anyone is kind enough...
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Basic data extraction on bam files
I'm trying to get read counts for things like reference normal forward/reverse count, alternative/reference forward/reverse counts from a bam file. I've...
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Raw read count to reference genes
I set a my own reference database containg selected genes (n=1500).
I had a 454 data about 100MB.
I run Bowtie 2 to get SAM, sorted/indexed....
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Different read counts
Hello all,
I am Velmurugan from India working on the role of miRNAs during environmental chemicals accumulation in mammals. In this regard,...
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Subsampling bam files
Hello,
I need to extract reads from a bam file - for each genomic position, I need to extract a different number of reads.
...
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summarizeOverlaps vs countOverlaps
Dear All,
I am getting completely different results for the count of mapped reads on transcript features using the countOverlaps...
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Counting reads (not coverage...)
Hi
Hopefully someone knows of some software or a script that will save me having to write oneā¦.
I want to put together some summary...