Hello,
We've done 16S/18S/ITS/amplicon sequencing for years, with good results - sometimes excellent (library dependent) - for both internal...
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16S & 18S Deep Sequencing Request(s)
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Recommended read depth for low input bulk rna-seq
Hi,
I'm wandering what is the sequencing depth recommended for low inupt bulk RNAseq, let's say 1000 cells.
I found on the Immgen website...
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Calculating Read/Coverage Depth per Base Pair from a GFF3 File
Hey, this is my first post and have been searching for hours trying to find a clear answer to this but could not for the life of me. Any input would be...Last edited by dgosting; 10-12-2015, 10:14 PM.
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read depth analysis on WGS data stored as .sra from ncbi
greeting all,
We are currently interested in preforming read depth analysis in order to investigate a possible CNV in a loci on human chr...
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read depth, number of reads explanation
HI I am very new to sequencing and am trying to learn the basics for use on a miseq sequencer.
i understand that the read length is the...
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minimum and maximum CNV size by Read Depth?
Hello! everyone.
Read depth (RD) is a good method to detect CNV. Generally, CNV size by RD is not large as far as I know, also depends...
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CNV and Depth of coverage statistical method
Hi,
I have depth of coverage file generated by GATK having depth of coverage for 20 samples on each chromosome. I am interested in comparison between...
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Read Depth in vcf (samtools / bcftools)
I generated one vcf file from 4 different bam-files (4 samples) and had a look at some variants in the vcf and in the IGViewer (IGV).
I...
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SAMTOOLS SNP calling question
Hi,
I have used tophat to map my paired-end Solexa reads to the mouse genome. I have called the SNPs using samtools and below is an exert...