16S & 18S Deep Sequencing Request(s)

cement_head

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Join Date: Mar 2012

Posts: 264
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#1

16S & 18S Deep Sequencing Request(s)

08-04-2020, 08:40 AM

Hello,

We've done 16S/18S/ITS/amplicon sequencing for years, with good results - sometimes excellent (library dependent) - for both internal and external clients.

We're starting to see more and more requests for guaranteed extremely high read depth per sample - like in the neighbourhood of 50,000 to 100,000 reads per side per PE sample. What kind of Illumina instrument (other than a MiSeq) does this (amplicon) sequencing well and why are people becoming obsessed with ultra-high read depth?

TIA
Tags: 16s, amplicon sequencing, illumina, miseq, read depth
KevinLam

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Join Date: Nov 2009

Posts: 204
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#2

08-04-2020, 09:47 PM

Hmm e-dna?
environmental DNA?
deep metagenomics?

I think Ion Torrent does a fairly good job of this ..

http://kevin-gattaca.blogspot.com/
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16S & 18S Deep Sequencing Request(s)

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