62 results in 0.0026 seconds.
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Viral Genome reconstruction
Hi! I have some reads from sequencing a viral genome. We are analyzing readings with two matches, and we calculate the existing distance between both... -
Is a compositional bias in Sequence Content of initial bases in WES reads normal?
Per Base Sequence Content
Any experienced Whole Exome Sequencing analyst/experimentalist:
the Per Base Sequence Content... -
Question: Not-Merged Reads What to do?
Hey guys,
So I'm working with pair-end data and I'm concerning regarding the merged/not-merged reads. I'm currently merging them using PEAR and... -
Pooling libs. for Novaseq. How many reads/coverage?
Hi,
I need to pool 10 multiplexed libraries with different insert sizes (from 245 to 285 bp). Two of the libraries are inputs. The rest... -
Working with BAM files
Hi everybody!
This is my first thread in this forum.
Recently, I started an internship in a bioinformatics research group. Unfortunately,... -
Assembly problem
hello everyone.
I am going to work on velvet assembler but my sample does not have two files for input(only forward reads are available).... -
amount of reads
How does lowering the amount of reads in RNA-seq or in resequencing experiment different in the way it effects the results? Does it affect these two application... -
TristanB started a topic Compare avg fragment length of major/minor allele reads at heterozygous positionin GeneralCompare avg fragment length of major/minor allele reads at heterozygous position
Hi all,
I would like to compare average fragment length among reads contributing to a minor allele at a given heterozygous position vs... -
All ChIP Seq Reads failing to Align to reference genome
Hello NGS community,
I'm new to NGS analysis. I have some chipseq data for a transcription factor. The sequencing facility provided data as CRAM... -
Duplicate read names - BWA mem - paired reads have different names
Hi,
running BWA mem (- PE; - Illumina), I'm getting the following error (replaced the ids):
[mem_sam_pe]... -
NextSeq reads count
Hi all,
I counted the number of reads in the same sample in 2 different ways:
a) On the sequencer (NextSeq), by counting... -
Tophat mapping problem on reads with length=50nt(or 51nt)
Hi,
I used tophat version 2.0.10 to map a fastq file with reads (=51nt) to human reference genome with the following command:
tophat... -
How can i get the number of read aligned to one scaffold
Hi.
I want to get the number of reads aligned to one scaffold.
more specifically,
I want to get the number of reads that... -
Reads do not align with reference genome
Hello community.
I am new to NGS world and I'm facing some problems while trying to align my reads with a reference genome.
... -
Understanding 16s RNA reads
Hello Members,
Thanks for your extensive support.
I'm completely fresh to 16s RNA, amplicon sequencing. I come from this background....
