Dear all,
recently I wrote a code to work with DRAGEN and RNAseq pipeline.
I use this command:
> /opt/edico/bin/dragen...
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Problem with DRAGEN RNAseq hashtable directory
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how to become a reviewer for Bioinformatics journals?
I would really like to offer peer-reviewing for the Bioinformatics/computational biology/cancer research journal or conferences. Anyone here have suggestions...
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chr20 length problem
Hi,
I have an Abi SOLiD WGS csfasta reads. After QC and filtering steps, I aligned the reads via SHRiMP2, specific for csfasta reads. I used hg19...
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Pathogen and Probiotics database of 16S rRNA
Hi all,
I want to use 16S rRNA reference that has clinical information such as pathogenetic and probiotic information.
What is the...
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1000 genomes phase 3 reference
Does anyone know what reference data they used for alignement in phase 3, 20130502 release?
Browsing through their ftp I have only found phase...
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Formatting a custom genome reference
Hi All,
I have a transgenic mouse whose genome I've sequenced and I'm interesting is answering a few questions concerning insertions,...
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indexing the nt database with makembindex to improve blastn (task megablast) runtime
I am trying to dust mask the NT database and then to index it using makembindex. However, when I run megablast on the indexed and dust masked nt database...
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Reduce .fa reference file
I am using the "BWA for SOLiD" tool on Galaxy. It calls for two inputs:
1) "Reference Genome": I am using mrna.fa.gz...
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[PICARD] Problems with SAM/BAM files without header
Hi there,
I have several problems when dealing with SAM/BAM files without an appropriate header inside. First of all, if there is a correct...
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Samtools output errors (ref is all 'N' in vcf)
I am working on using bowtie and samtools to take a fastq file, map to HG19 using bowtie, and use samtools to find SNPs and output a VCF file.
...
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Creating bacterial reference genomes
Dear all,
I would like to create two complete bacterial genomes from two recently described bacteria. I have draft genomes for both. My...
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1000 genomes human reference
Hello,
I'm using hg19.
As you may know when you perform variant calling, reference variation allele may be biased due to...