Dear all,
recently I wrote a code to work with DRAGEN and RNAseq pipeline.
I use this command:
> /opt/edico/bin/dragen -f -l \
> -r refdir \
> -1 ${forward} \
> -2 ${reverse} \
> -a ${gtf} \
> --output-dir output/${sample} \
> --output-file-prefix ${sample} \
> --RGID ${sample}_group_id \
> --RGSM ${sample} \
> --enable-rna true \
> --enable-rna-gene-fusion true
I get back a this error:
> ERROR: The specified hashtable directory cannot be used to run RNA: refdir
> Regenerate the hashtable with "--build-hash-table true" and "--ht-build-rna-hashtable true"
The problem is that my refdir was generated with this flags, I've tried a lot of different workaround but the result doesn't change.
This is the command that I've used to build the has table:
> Automatically generated by dragen version 01.003.044.4.0.3.
> Command line: /opt/edico/bin/dragen --ht-reference refdir/ref_genome.fa --output-directory output/ref --build-hash-table true --ht-build-rna-hashtable true --ht-mask-bed /opt/edico/fasta_mask/hg38_alt_mask.bed
> Hash table version 8
I really have no clue about what could it be the error, I hope to find someone that has my same issue.
Thanks in advance!
Best regards,
Youssef
recently I wrote a code to work with DRAGEN and RNAseq pipeline.
I use this command:
> /opt/edico/bin/dragen -f -l \
> -r refdir \
> -1 ${forward} \
> -2 ${reverse} \
> -a ${gtf} \
> --output-dir output/${sample} \
> --output-file-prefix ${sample} \
> --RGID ${sample}_group_id \
> --RGSM ${sample} \
> --enable-rna true \
> --enable-rna-gene-fusion true
I get back a this error:
> ERROR: The specified hashtable directory cannot be used to run RNA: refdir
> Regenerate the hashtable with "--build-hash-table true" and "--ht-build-rna-hashtable true"
The problem is that my refdir was generated with this flags, I've tried a lot of different workaround but the result doesn't change.
This is the command that I've used to build the has table:
> Automatically generated by dragen version 01.003.044.4.0.3.
> Command line: /opt/edico/bin/dragen --ht-reference refdir/ref_genome.fa --output-directory output/ref --build-hash-table true --ht-build-rna-hashtable true --ht-mask-bed /opt/edico/fasta_mask/hg38_alt_mask.bed
> Hash table version 8
I really have no clue about what could it be the error, I hope to find someone that has my same issue.
Thanks in advance!
Best regards,
Youssef