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  • yussab
    Junior Member
    • Jan 2024
    • 1

    #1

    Problem with DRAGEN RNAseq hashtable directory

    Dear all,

    recently I wrote a code to work with DRAGEN and RNAseq pipeline.
    I use this command:

    > /opt/edico/bin/dragen -f -l \
    > -r refdir \
    > -1 ${forward} \
    > -2 ${reverse} \
    > -a ${gtf} \
    > --output-dir output/${sample} \
    > --output-file-prefix ${sample} \
    > --RGID ${sample}_group_id \
    > --RGSM ${sample} \
    > --enable-rna true \
    > --enable-rna-gene-fusion true

    I get back a this error:

    > ERROR: The specified hashtable directory cannot be used to run RNA: refdir
    > Regenerate the hashtable with "--build-hash-table true" and "--ht-build-rna-hashtable true"

    The problem is that my refdir was generated with this flags, I've tried a lot of different workaround but the result doesn't change.

    This is the command that I've used to build the has table:

    > Automatically generated by dragen version 01.003.044.4.0.3.
    > Command line: /opt/edico/bin/dragen --ht-reference refdir/ref_genome.fa --output-directory output/ref --build-hash-table true --ht-build-rna-hashtable true --ht-mask-bed /opt/edico/fasta_mask/hg38_alt_mask.bed
    > Hash table version 8

    I really have no clue about what could it be the error, I hope to find someone that has my same issue.
    Thanks in advance!
    Best regards,
    Youssef​

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