15 results in 0.0034 seconds.
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trouble shooting PB assembly generating a larger than expected contig
So I've got a newly assembled genome that was sequenced using PacBio sequencing and obtained >100x coverage. It was assembled with HGAP.3 and for the... -
Best approach for targeted PCR resequencing on MiSeq from RNA
Hi everyone,
I need to amplify selected regions from my RNA samples and resequence with MiSeq.
I am unclear to what is the... -
MiSeq workflow - tissue to instrument
Our lab is new to NGS and we are in the midst of planning our first sequencing run. Our project involves targeted resequencing of 15 horse genes, about... -
2 strains.De novo or resequencing?
Hello,
I am interested in sequencing 2 related strains of a Gram Negative bacteria, one sensible and the other resistant to multiple antibiotics... -
Newbie dummy question, please help
Hi, folks:
I am doing research on Arabidopsis. The existing reference genome is on one ecotype (Col), and we have sequenced another ecotype (Ws),... -
Peculiar crash in BWA samse
Hi all:
I have a bash script which manages our analysis pipeline to do assembly of multiple sequences. It runs without trouble on a Mac... -
Hi there!
I'm new to this site, and fairly new to molecular biology and next-gen platforms. I'll be sure to post some good questions!
Paul -
Minimum dsDNA detection by NGS machine
Hello,
I am new here and to NGS. I prepared some multiplex DNA libraries with Illumina TrueSeq Kit. I submitted each sample/index at 10nM,... -
Removing Duplicates Scenario Exome Resequencing
I am rethinking my well-established pipeline and trying to tweek it a bit and came across some controversy on the use of remove duplicates. I would like... -
wet lab Validation of a large number of SNPs
Hi
AFAIK
you can validate SNPs obtained by Hi-seq by
microarray,
Sanger Seq,
NGS seq on a different platform ... -
Crossbow alignment format
I am using crossbow v1.1.1 on a local computer cluster to map a set of human SOLiD reads and call the variants. Crossbow glues together the bowtie aligner... -
Duplicates percentage in target resequencing
Hi,
We are performing a target resequencing experiment, where we enriched 8 regions.- What's the mean % of duplicates in this kind of
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No output from samtool's mpileup
I am trying to run samtools for SNP detection, but neither mpileup or the deprecated pileup produces any output.
My data are in SAM format,... -
high Q ambiguous SNPs from Maq
Hello,
I'm doing mutation detection by ~30x Illumina genome resequencing on a haploid eukaryote.
Maq seems to be working fine... -
Amplicon sequencing: Method to prevent end over-representation & improve uniformity
Thought people would be interested in this!
Biotechniques. 2009 Mar;46(3):229-31.
Method for improving sequence coverage uniformity...
