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Can someone explain how Ribo Zero works?
I'm using the Ribo Zero Magnetic Kit from Epicentre and I get that somehow my rRNA ends up bounds to the beads and my ribo-depleted RNA is in the supernatant... -
Low RIN of plant RNA and high abundaces of rRNA.
Hi there and thanks in advance for your input!
We extracted total RNA from (plant) leaves, using the quiagen RNeasy kit. As expected, we... -
quantification of rRNA and tRNA content in RNA-seq library
Hi all,
does anybody has a tested procedure to quantify the number of reads that map to either rRNA or tRNA in RNA-seq library?
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rRNA identification tools or information PLEASE!
Hi everybody,
I identified in my genome (by blast) 1 contig which is the rRNA genome.
Now, I am searching for tools able... -
Unplaced chromosomes / supercontigs during mapping
Hi all,
when you download the human reference genome it usually includes many different kinds of unplaced supercontigs such as GL000217, GL000218,... -
multiple rRNA peaks Biorad Experion
Dear All,
While testing my RNA samples for quality on the BioRad Experion i noticed multiple peaks before the 18S peak. The pattern is... -
How much rRNA sequence is reasonable in an RNA-seq dataset?
Dear all,
I have just received two RNA-seq datasets from a bacterial comparision. The reads are at least 50% (have not been able to look deeper... -
Filter ribosomal RNA
Hi
Although ribosomal depletion is usually performed some sequences with ribosomal content remain and are sequenced.
How...
