Can someone explain how Ribo Zero works?

[SS00]

I'm using the Ribo Zero Magnetic Kit from Epicentre and I get that somehow my rRNA ends up bounds to the beads and my ribo-depleted RNA is in the supernatant but I am not sure on the specifics. Can anyone help with this? Specifically: -

- What is in the ribosomal removal solution. Are they oligos that are specific for ribosomal RNA?

- I have found a paper mentioning that the ribo zero kit uses a hybrid substraction technique but not sure what this is?

- Why does my ribosomal RNA end up bound to beads but the rest doesn't?

[kmcarr]

I'm using the Ribo Zero Magnetic Kit from Epicentre and I get that somehow my rRNA ends up bounds to the beads and my ribo-depleted RNA is in the supernatant but I am not sure on the specifics. Can anyone help with this? Specifically: -

- What is in the ribosomal removal solution. Are they oligos that are specific for ribosomal RNA?

- I have found a paper mentioning that the ribo zero kit uses a hybrid substraction technique but not sure what this is?

- Why does my ribosomal RNA end up bound to beads but the rest doesn't?

Check the Ribo-Zero FAQ, specifically #9 and #14, and a better description is hybridization/capture not hybrid subtraction.

[SS00]

Check the Ribo-Zero FAQ, specifically #9 and #14, and a better description is hybridization/capture not hybrid subtraction.

Brill, thanks!

[Phage434]

For a detailed description of protocols, see the patent application US 2011/0040081 A1
Methods, Compositions, and Kits for Generating rRNA-Depleted Samples or Isolating rRNA from Samples, Roy. R. Sooknanan, assigned to Epicentre