Hi everyone,
This is my first time in this area of research. I am working on 95 varieties of bambara groundnut. I have done the agromorphological...
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SNP calling from RNAseq data
Hello NGS fellows,
I am a newbie here and would highly appreciate your advice about one particular experimental design.
...
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amount of reads
How does lowering the amount of reads in RNA-seq or in resequencing experiment different in the way it effects the results? Does it affect these two application...
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RISC effect to mRNA during RNA-seq preparation
Hello,
I have a question regarding the RISC (RNA induced silencing complex, especially miRNA) and RNA-seq. I'm not a biologist so forgive...
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Useful to run total RNA along with RIPseq?
I have some RNA isolated from an RNA immunoprecipitation. I was wondering if I should also sequence the (input) total RNA and what advantage/use this...
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TopHat -M/--prefilter-multihits; unmapped reads
hi!
i am confused when to use TopHat "-M/--prefilter-multihits and when not
"When mapping reads on the transcriptome,...
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DESeq2 design error
Hi,
I am trying to run DESeq2 using the "Differential analysis of count data ~ the DESeq2 package provided in the bioconductor website....
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RNA seq expt design
Hello,
I am trying to design an experiment :
and my question is can I put together my 3 reps of RNA before doing the library and...