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A Basic Guide to RNA-sequencing
Next-generation sequencing (NGS) is the modern, second-revolution and the spearhead of an ever-accelerating field. The NGS technology performs innovative...
Last edited by Novogene; 07-25-2024, 12:48 PM. -
Circular RNA Sequencing
Hello Everyone, we aim to study Circular RNA in a few days. Can you give advice for optimization and Library Prep phase? I will be using the Illumina...Last edited by MehmetZeki; 01-26-2024, 08:24 AM. -
EMBL-EBI on-site course | Introduction to RNA-seq and functional interpretation
EMBL-EBI on-site course | Introduction to RNA-seq and functional interpretation | 20 – 24 February 2023.
Gain an introduction to the technology,... -
splitting Tophat2 bam files in separate samples
Hello everyone,
I have RNA seq data of 12 samples (paired end, so 24 files) and I ran tophat2 for alignment on all of them which gave... -
Odd sequences in RNAseq fastq
Hi all,
Recently, I just ran iSeq for pair ended RNA sequencing and it gave me odd fastq results
Some of Read1(i7) sequences have...Last edited by Kujin Kwon; 11-20-2019, 02:39 AM. -
high rRNA after poly(A) selection
Hi,
About half our samples post library prep (poly(A) selection) + sequencing had around 50% rRNA content. The samples were re-prepped... -
ht seq counting/ Most of reads into alignment non unique
Hi all,
I am new to Rna seq. I am runnign ht seq within galaxy but at the end most of my reads end up in alignment non unique
... -
Removing overrepresented sequences (rRNA contaimation) from RNAseq raw data
Hello,
I recently got data back from a single-end RNAseq run. After looking at the fastQC report, there were many overrepresented sequences... -
Fragment length for expression estimation
I have sequenced using Illumina and quantified with Salmon. I have run some QC plots and have what looks like a spurious sample (see attached)
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Statistical analysis of Centrifuge output
What type of statistics should I use to analyze output from metagenome classifier "Centrifuge"?
I have run Centrifuge on 24 samples from... -
FeatureCounts highest counts for unstranded option
Dear All,
I am working on RNASeq data which is generated using Truseq straded HT kit.
I have done removing adapters and... -
FeatureCounts highest counts for unstranded option
Dear All,
I am working on RNASeq data which is generated using Truseq straded HT kit.
I have done removing adapters and... -
Tophat2 alignment problem
I ran this code using tophat (v2.1.0) to align reads (bowtie2 (v2.2.6.0)) from my RNA-seq fastq file using the bowtie2 genomes.bt2 indexes from igenomes... -
de novo metatranscriptomics pipeline for soil
Hi all, I'm trying to figure out a good pipeline for a de novo metatranscriptomics project on soil and rhizosphere samples. I'm looking into the Khmer... -
Webinar: How one researcher analyze piRNAs from 7,000 TCGA sample
Webinar:
Unique somatic and malignant expression patterns implicate PIWI-interacting RNAs in cancer-type specific biology
April...
