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Roche NimbleGen number of bases
I have 5 samples that were sequenced with SeqCap EZ Human Exome Library v2.0 kit from Roche/NimbleGen. I ran it with BWA for alignment and converted to... -
Roche 454 RL adapters.. help!!!
Hi,
We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We... -
RNA-Seq: TopHat with Roche/454 reads
Hellow
I have got Roche/454 RNA-Seq reads from human samples.
It seems that TopHat/Cufflinks is one of the most used pipeline for... -
Problems with 454 GS FLX Titanium medium volume sequencing
Hi,
I've been running my first ever pyrosequencing reactions recently, using all the kit and instructions provided by Roche for the GS... -
PANGEA (Pipeline for analysis of next generation amplicons)
I wanted to give you all the heads up on a new paper published in The ISME Journal this month (4, 852-861, July 2010), on a program for sorting and assembling... -
Bead preparation for "GS Junior" with ABI EZ Bead Dystem
Hello!
If we would have and Roche "GS Junior" and also the Applied Biosystems (Life Technologies) "EZ Bead Sytem" (SOLiD... -
best primers for 16S amplicon sequencing
Hello All
We are starting a metagenomics project to identify mixed bacterial populations in various biological specimens. Any suggestions for robust... -
gsMapper: how to reduce number of gaps ?
We would like to ask whether anyone else has seen the same problems when using 454's gsMapper with a set of reads and a reference sequence. How can one... -
Roche/454 for bacterial ChIP DNA
Hi,
I work with bacterial transcription factors.
I am trying to find out alternatives to ChIP-chip and am considering 454... -
Roche 454 + Illumina/Solexa hybrid assembly strategies
Hi, all.
I'd like to know what strategies people are using to perform hybrid 454/Illumina assemblies. What general approach are people...
