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Hi,
We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
Thank you,
Vartul
We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
Thank you,
Vartul
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