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Roche 454 RL adapters.. help!!!

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  • Roche 454 RL adapters.. help!!!

    Hi,

    We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
    Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
    Thank you,
    Vartul

  • #2
    This technical bulletin should help answer your questions.
    Attached Files

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    • #3
      Dear RCJK, many thanks for the technical bulletin.

      Comment


      • #4
        please give me this technical bulletin

        Originally posted by Vartul View Post
        Dear RCJK, many thanks for the technical bulletin.
        Dear sir, can you give me this technical bulletin?thank you so much.

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        • #5
          Hi!
          could you upload this technical bulletin again?
          I cannot download it from the link you provided already a decade ago...
          Thanks!
          Ellli

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