Hi all,
We ordered cassettes for our Blue Pippin back in March and are still getting the run around from the company (https://sagescie...
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Sage Science Blue Pippin Supplies unavailable
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Why A and B primers in SAGE? Why not just one?
Reading about SAGE (serial analysis of gene expression). Everywhere discusses adapters having A or B primer but no one explains explicitly why dividing...
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Bimodal distribution in TruSeq libraries
Hi,
I made some control TruSeq genomic gel-free libraries using Ecoli and pooled these together equimolar. This pool then underwent blue pippin...
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Deep Sage for Plants with no genome-Good approach?
Given that there's alot of plant genomes out there.
Is it a good idea to use Deep SAGE to do gene expression profiling for a plant with no reference...
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HiSeq versus Solid for DGE
Dear All,
this is my first post.
I have to make purchasing decision for a sequencer for the my lab in the next two months.
My need...
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SOLiD: TooFewCalls
Hi
I did a SOLiD 3plus run (SAGE experiment).
In the stats-file the following lines were found:
...
# File:...
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Alignment of Long SAGE tags
hello all,
I've just started doing sequence alignment. Interested in aligning 21bp Long SAGE tags.
What are the software packages available,...
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Gene Expression Tag Table Software Now Available
I've had several of requests to make our tag table creation software publicly available, so now it is.
http://research.stowers-institute...