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Utilizing Innovations in Single-Cell Sequencing
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Dam-ID prep
Hi all,
I recently sequenced my Dam-ID library. Unfortunately for some reason, I found a lot of read-missing regions even around known... -
troubleshooting agilent bioanalyzer error
I ran an RNA 6000 nano chip with RNA samples in all 12 wells, some randomly came back flagged with error: unexpected signal in fast region. Is there any... -
Covaris M220 service plan
Hi all,
Our department is considering purchasing a Covaris M220 so we can begin generating libraries in house. A one year service plan... -
sample prep kit recommendation?
Hi I am new to NGS. and I have read a lot from your posts. thanks first.
we plan to do NGS from dsDNA for target exome sequencing. the... -
Cheapest cell lysate --> mRNA strategy
Hi,
Can anyone fill me in on the most cost-effective strategy from going from cell lysates to mRNA, either directly or indirectly? Currently,... -
Nextera XT
Hi all,
Does anyone have experience/success using the Nextera XT kit with reduced reaction volumes? I know you can setup 1/10 reactions... -
DNase treatment and fragmentation of RNA
Hi all,
I have recently started RNA extraction on different fish tissues with the aim of carrying out RNA-seq using Illumina Hi-seq.... -
Cutting corners with Illumina Sample Prep
Hey all, just a quick question regarding Illumina's TruSeq DNA sample prep protocol. After adapter ligation, there's a cleanup step with Ampure XP. If... -
Pippin Prep for RNA?
For those of you familiar with the Pippin Prep, currently used for automated DNA size selection and band capture, your thoughts on whether or not Sage... -
High AT dropout
Hi
i am running SCS2.9 on Illumina GAIIx, recently i noticed AT dropout to some of my libraries.All the libraries are made with same procedure,... -
What is the sample prep like for 454?
Hi Folks
What is the sample prep time for genomic and transcriptomic libraries for 454?
What specialized equipment do you need for library... -
Chip-seq sample prep for bacteria
I want to do chip-seq for a transcription factor in bacteria for Illumina. I' need help for sample preparation for bacteria since all I find is eukaryotes.... -
Roche/454 for bacterial ChIP DNA
Hi,
I work with bacterial transcription factors.
I am trying to find out alternatives to ChIP-chip and am considering 454... -
Hello from NuGEN Technologies
Steve Kain here. We have recently introduced a solution for RNA-Seq sample prep with inputs of total RNA in the range of 500 pg to 100 ng. The product...
