Hello
I have environmental cDNA left over from a tricky qPCR project. Is it possible to use Sanger Sequencing of the ITS 5.8S rDNA region to observe...
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Sanger Sequencing for Community Analysis
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Problems installing bcftools and fixref plugin for using Sanger Imputation Service
Dear all,
I am trying to use the Sanger imputation service (https://imputation.sanger.ac.uk/), but I get the following error:
"Reference... -
Het/Mixed Indel identification tools
I have sanger data (.ab1 files) that i want to call het/mixed indels on, i was wondering what are some of the tools you all use for such analysis. So... -
direct sequencing vs sequencing of cloned PCR product
Dear all,
I would like to know which of the two is better, sequencing PCR product directly or sequence the PCR product after cloning... -
How to perform grooming that galaxy does but on the commandline?
How do I groom sanger reads into sanger reads on my local commandline using the same tool that galaxy has found here?
In other words,... -
Comparing metagenomes produces with different sequencing technologies
Hi all,
I would like to compare the abundance of my gene(s) of interest across many published metagenomes. Typically we would normalize... -
Formamide problems?
I've accidentally added formamide to my mixture before PCR (tube contained template, primer, polymerase...) I had mistakenly grabbed a tube of formamide... -
Matrix Creation Error
Hi,
We are using the sequencer ABI Sanger 310 and we have problems with the sequencer.
When made the Matrix, this send us the following... -
strains identification by STR micrsatellite using NGS
We have many vials that contain mixture of yeast STRAINS (of 3 or more strains). Currently we are using Capillaries Electrophoresis (CE). With culturing... -
Dr. Fred Sanger has passed away
This is a sad day indeed.
BBC is reporting that Dr. Frederick Sanger has passed away at the age of 95.
He is the only Briton...Last edited by GenoMax; 11-20-2013, 05:55 AM. -
Why Sanger sequencing can have the forward and reverse reads?
I read the page and get to know Paried end reads are most oriented to the NGS, like Illumina aiming to get longer sequencing read.
ht... -
(newbie question) What is the diff on shotgun method and the other methods?
As in the title.
What is the diff on the shotgun sequencing method and other methods (Sanger, or NGS)?
Thanks. -
Is Sanger better than Next-Gen for Anything?
Do you think there are any applications for which Sanger sequencing (i.e. capillary electrophoresis, CE) still holds a competitive advantage over Next-Gen... -
Mutations detection
Hi all,
I know that maybe there's many threads that talked about a pieces of what I'm going to ask, but I'm really in a none issue situation... -
Validation of Illumina on other NGS platforms
Hi we are currently doing our Illumina variant validations using sanger sequencing. Has anyone used another platform like 454 or PGM to validate their...
