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(newbie question) What is the diff on shotgun method and the other methods?

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  • (newbie question) What is the diff on shotgun method and the other methods?

    As in the title.

    What is the diff on the shotgun sequencing method and other methods (Sanger, or NGS)?

    Thanks.

  • #2
    A bit of searching would have landed you here: http://en.wikipedia.org/wiki/Shotgun_sequencing

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    • #3
      Originally posted by arkilis View Post
      As in the title.

      What is the diff on the shotgun sequencing method and other methods (Sanger, or NGS)?

      Thanks.
      I think you are confused about some basic terminology. Shotgun is a sequencing strategy. Sanger or NGS are sequencing methodologies (though "NGS" is a generic term which encompasses several different technologies). The shotgun strategy may be carried out using many different methodologies/technologies, including the two you have listed.

      GenoMax is correct. There are several very good introductory tutorials about sequencing strategies and technologies a mere Google search away. It wouldn't be useful to simply repeat here what is already well described elsewhere.
      Last edited by kmcarr; 09-23-2013, 07:54 PM.

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      • #4
        Originally posted by GenoMax View Post
        A bit of searching would have landed you here: http://en.wikipedia.org/wiki/Shotgun_sequencing
        Thanks, I went to Shotgun wiki page. So many terminologies get me lost there...

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        • #5
          Originally posted by kmcarr View Post
          I think you are confused about some basic terminology. Shotgun is a sequencing strategy. Sanger or NGS are sequencing methodologies (though "NGS" is a generic term which encompasses several different technologies). The shotgun strategy my be carried out using many different methodologies/technologies, including the two you have listed.

          GenoMax is correct. There are several very good introductory tutorials about sequencing strategies and technologies a mere Google search away. It wouldn't be useful to simply repeat here what is already well described elsewhere.
          Thanks a lot. The answer is quite clear and straightforward.

          Comment

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