Hi all,
Is it possible for contig and scaffold N50s to be very similar (e.g. within 2-3k bp) or the exact same? I have seen a few examples...
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Contig and scaffold N50
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How to know if a SNP is non synonymous using the genome sequence and the gff3 file?
Hi everyone,
Everything is in the title: How to know if a SNP is non synonymous using the genome sequence and the gff3 file?
I have no other...
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Samtools: extract multiple cromosomes/scaffolds from bam file
Hi,
I want to create a bam file containing three or four chromosomes/scaffolds.
I only know this command
samtools view -b...Last edited by Anti; 01-12-2015, 03:10 AM.
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454 ACE assembly with paired reads... how to view?
Hi,
I'm new here, thanks in advance for any help.
I have a 454 dataset of 'allcontigs.ace'. The reads consist of paired and...
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export scaffold files from SAM/BAM alignment
Using BWA, I have aligned ~10M MiSeq paired end reads to ~100 sequence contigs obtained from non-paired end 454 data. Most of the contigs remained intact...
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Scaffolding tool
Do you know any scaffolding tool targeted to NGS plattaforms? I am looking for something that can use contig fastas and aligned paired ends (eg SAM format)...
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Size of the reference sequence for SAET
Dear all,
This is my first post to this extremely useful forum about NGS (congratulations to everybody, and specially to the creators and...