Header Leaderboard Ad

Collapse

Contig and scaffold N50

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Contig and scaffold N50

    Hi all,

    Is it possible for contig and scaffold N50s to be very similar (e.g. within 2-3k bp) or the exact same? I have seen a few examples of this in some de-novo assemblies that I am working with and just want to make sure this is OK.

    Thanks for any thoughts!

  • #2
    PacBio assemblies will have contig and scaffold stats that are very close. I guess single-end Illumina or paired-end Illumina on short fragments would also be like that?
    Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

    Comment


    • #3
      I am using paired end Illumina reads. Usually I see that the contig N50 is 1/2 or 2/3 of scaffold N50, so I was a bit concerned when we got some with exactly the same contig/scaffold N50

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
        by seqadmin



        Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
        03-21-2023, 01:49 PM
      • seqadmin
        Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
        by seqadmin




        Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
        03-10-2023, 05:31 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-24-2023, 02:45 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2023, 12:26 PM
      0 responses
      13 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-17-2023, 12:32 PM
      0 responses
      17 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-15-2023, 12:42 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Working...
      X