To score a sequence alignment, the distribution of local alignment scores involving random sequences is used as a statistical reference.
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Techniques for estimating local alignment score distribution parameters
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Primer3 primer dimer score
I have used Primer3 tool within Geneious to design a large number of primers. There is one parameter that I would like some help in interpreting. The... -
BWA change mismatch penalty between N-mismatch and base-mismatch
Dear all
I am trying to use BWA with different penalty score between N mismatch and a base mismatch [ACTG]. Is there any software option that fits... -
Per Base Quality scores in FastQC
Hello,
I'm very new to analysing next gen data, and I was hoping someone might be able to help me interpret a failed diagnostic plot I've... -
BWA FASTQ input score type
Does BWA expect phred-33 or phred-64 (or something else) type quality scores for input? -
Illumina FASTQ Quality Scores - Missing Value
Hi there,
I'm having a look at some FASTQ files generated from the Illumina GA pipeline (I think version 1.3). The data is for a series...
