I have used Primer3 tool within Geneious to design a large number of primers. There is one parameter that I would like some help in interpreting. The primer dimer score, which ranges from 0 to a large number of some kind, is somewhat confusing. How is this calculated? What is considered a “good” number for primer dimers not to form? Obviously zero is ideal, but when I look at my dataset, It ranges up to 30 or so, with everything in between. The online help information gives examples of 6 or 7. I would appreciate any laboratory experience advice on what would be a good threshold to avoid. I am developing primers for Fluidigm/Illumina. Thanks!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM