I have used Primer3 tool within Geneious to design a large number of primers. There is one parameter that I would like some help in interpreting. The primer dimer score, which ranges from 0 to a large number of some kind, is somewhat confusing. How is this calculated? What is considered a “good” number for primer dimers not to form? Obviously zero is ideal, but when I look at my dataset, It ranges up to 30 or so, with everything in between. The online help information gives examples of 6 or 7. I would appreciate any laboratory experience advice on what would be a good threshold to avoid. I am developing primers for Fluidigm/Illumina. Thanks!
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