Hello people,
I tried the ScriptSeq v2 RNA Library Prep Kit on the total RNA extract of a cerebrospinal fluid sample. The RNA amount was...
9 results in 0.0021 seconds.
-
ScriptSeq v2 135base-peak
-
Seeing reads in both directions from a directional library?
I made bacterial RNA-seq libraries with ScriptSeq v2 and did a small sequencing test (MiSeq Nano, Single Read, 50 bp).
ScriptSeq v2 prepares... -
Scriptseq insystem contamination
I have recently begun using the scriptseq complete gold epidemiology kit to sequence RNA from human blood samples. From the number of runs I have done,... -
Scriptseq complete gold epidem
I have total RNA extracted from environmental samples that I intend to sequence. The main aim is to detect viral and bacterial transcripts.
... -
How to deplete RNA without RiboZero or Terminator exonuclease?
Hello everyone!
Thank you for reading my question! Please excuse my little experience in the topic, I would like to have some advice on the RNA-seq... -
ScriptSeq - 2nd peak @ 550bp
Hi
I have been preparing libraries from approx 400ng total RNA, mRNA enrichment, using the ScriptSeqV2 kit.
At the end I amplify with 12... -
casava 1.8.2 adapter masking new option
Hello,
I am starting to work with casava 1.8.2 for demultiplexing data with samples with dual and single index in the same paired-end Illumina... -
default RNA-Seq library prep
What with all the developments in the RNA-Seq library prep field our lab is contemplating moving away from the Illumina Tru-Seq RNA kits and using the... -
% rRNA remaining after polyA selection
Does anyone have a standard or threshold of % rRNA they require in order to proceed with mRNA library prep?
In the past I've only proceeded...
