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16S vs shotgun
Have people done detailed comparisons of bacterial communities profiled using amplicon (e.g. 16S) versus shotgun sequencing? I know HMP has done this...
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Shotgun Metagenomics data analysis
Hi,
I am new to shotgun metagenomics analysis. I have a project on the human gut microbiota. I know about MG-rast but I want to analyze...
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Filter 18s sequences using HMMs?
Hi all,
Apologies if this has been covered before but I'm fairly new to this and can't seem to find an answer to my question anywhere,...
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Suspiciously low read numbers with shotgun pipeline compared to amplicon pipeline
Dear all,
A company sequences our 18S amplicons and usually use the shotgun pipeline which usually yields more reads than the amplicon...
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FLX+ Library Amplification Issue
We are having problems getting our long-fragment shotgun library to amplify during emPCR. Titration using 3 different cpb ratios (12, 18, and 24) yields...