Hello guys,
I have some issues to understand the soft-clipped reads. I am analyzing a targeted resequencing panel. I trimmed the FASTQs, but I found...
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remove soft-clipped bases
I need to conduct some computations using a python script directly on some BWA aligned bam files.
For this, I need to remove the soft clipped bases....
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How to soft-clip in SAMTOOLS?
Hello everyone,
I am using SAMTOOLS to view, sort, and index parts of a patient's DNA. But no matter what I do, I always get the same answer...
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Soft-clipped alignments - keep or filter out?
I have question regarding the post-processing of RNA-Seq data. I'm using GSNAP version 07-03 for alignment of paired-end data and am getting a lot of...
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bowtie2 read length in score-min
I am trying to use bowtie2 in local mode to allow soft-clipping.
The parameter "score-min" lets me define a read-length dependent function...
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BWA Soft Clipping
Hi,
When I run BWA without specifying a "q" value (which defaults to 0 as I understand it from the manual), I would not expect...