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Analyzing PacBio sequel data and SRA
I am new to NCBI's SRA and pacbio sequencing data analysis. I am trying to download pacbio sequence data from SRA and my goal is get CCS (Consensus) reads...
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Downloading 'RunInfo Table' from SRA Run Selector
Hello,
I would like to download the metadata for a given BioProject from the SRA. I am able to get exactly what I need by hitting the download...
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NCBI SRA & TSA submission tutorial
We have just published a very detailed guide to submitting RNA-Seq reads to NCBI's SRA, and transcriptome assemblies to TSA. We have often felt the need...
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Does BAM file from SRA contain aligned reads?
Hello, everyone. I need to do SNP calling for the first time, so I have a couple questions.
For example, I want to use this sample https://tra...
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EBI Metagenomics: How to analyze NCBI data?
Uploaded and public data from NCBI-SRA is mirrored and accessible at ENA (European Nucleotide Archive).
EBI Metagenomics is a pipeline that analyzes...
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Query individuals with both WGS and RNA-Seq data
Using the SRAdb R package, I am looking to query individuals who possess both WGS and RNA-Seq data on the Sequence Read Archive. So far I believe I have...
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Single sample, more than one SRA file
Hello All,
I want to download some samples from the SRA (http://www.ncbi.nlm.nih.gov/geo/quer...i?acc=GSE22411) but sometimes a single...
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SRA issue
I am trying to use SRA to download public data SRR2129993. Running 'fastq-dump SRR2129993 -X 5 -Z' i get the output i expect - 5 spots read and write....
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Uploading PacBio raw data to ENA SRA
Surprisingly I was not able to find anything on Google about the details of uploading raw PacBio sequence reads to the European Nucleotide Archive (ENA),...
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SRA Metadata File - Sample Submission
Hi, I'm trying to submit WGS samples to SRA using the SRA Submissions Portal.
I'm stuck at the SRA metadata step of Biosample submission....
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SRA data in pileup format
Hello all,
I'm trying to use the first pileup file the below link points to:
http://trace.ncbi.nlm.nih.gov/Traces/sra/?study=SR...
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Casava FASTQ from SRA
Dear all,
I am currently trying to perform quality checks on a set of FASTQ files (downloaded from SRA) that (according to the authors)...
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Truncated File: Trouble with SRA > Fastq > Sam
Hi everyone,
I know there have been several posts regarding truncated SAM files, but I'm having a difficult time finding a solution....
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How do people know if a SRA dataset is generated by normalized cDNA library or not?
I am looking for RNA-seq data generated from non-normalized cDNA library. However, many datasets I have looked at do not show this information. How should...