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How indexes are added in TruSeq Custom Amplicon v1.5?
Hi folks. Could someone tell me that how indexes and sequencing primers are added in TruSeq Custom Amplicon v1.5 library preparation? By ligation (to... -
Cycle number? Indexing/enrichment NEBNext
I'm preparing dual-indexed libraries using NEBNext Ultra Library Prep for targeted resequencing on Illumina MiSeq and can't decide on the number of PCR... -
Webinar: Achieving Highly Accurate NGS Variant Calling
Join us on Wednesday, October 21 at 12:00 PM EDT (UTC -4:00) for a live webinar featuring:
- Discussion of accuracy and validation of NGS alignments
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Soft-clipping resequencing panel
Hello guys,
I have some issues to understand the soft-clipped reads. I am analyzing a targeted resequencing panel. I trimmed the FASTQs, but I found...Last edited by BnaT; 12-18-2014, 07:03 AM. -
CNVkit: Copy number detection and visualization for targeted sequencing using off-tar
CNVkit is a software toolkit to infer and visualize copy number from targeted DNA sequencing data. It is designed for use with hybrid capture, including...Last edited by etal; 02-04-2015, 09:41 AM. -
Targeted sequencing of ~40Mb genome
I am looking to do targeted sequencing on approximately ~40Mb of mouse genome (~2% of the genome) from low input DNA (~100pg). Equivalent to sequencing... -
The ratio of reads whose mate mapped to different chromosome in targeted sequencing
Hello all,
Recently my team sequenced targeted reads of tumor/normal pair, and the ratio of interchromosomal mapped reads looks weird.... -
Ion Torrent Sequence Analysis Webinar
Are you working with Ion Torrent data? Join us for a webinar next Wednesday, April 3 at 12 PM EDT (GMT - 4:00).
DNASTAR's Matt Keyser... -
Kits for targeted resequencing
I'm new to the world of targeted resequencing and would appreciate your help in understanding the options for performing our sequencing project within... -
Extracting reads with highest identity to my genes of interest from illumina raw data
I was wondering if there is a way to pull out the hits from illumina raw data according to a set of genes I am interested in rather than doing assembly... -
CONTRA test files available now
For those of you who have problems running CONTRA for copy number analysis over your exome / targeted resequencing data, I've now made a couple of bam... -
SOLiD Beads Sinking
Hi I am trying to perform some hybridization experiments, yet a problem I am facing is that the SOLiD beads are sinking and so I am unable to capture/enrich... -
Targeted resequencing - open source
Hi all,
Stanford Genome Technology has published a 2nd generation "open source" capture technology using in solution oligonucleotides.... -
Targeted Resequencing - Target file issues
Hello!
After an intensive month following getting my targeting resequencing data, and working with this kind of data for the first time, going... -
Exome Sequencing with NimbleGen SeqCap EZ v2
Hi all!
We used NimblGen's SeqCap EZ Human Exome Library v2.0 followed by paired-end sequencing on the Illumina GAIIx for exome sequencing....
