I'm new to the world of targeted resequencing and would appreciate your help in understanding the options for performing our sequencing project within our limited budget.
We would like to sequence ~50 non-coding areas in the human genome with length of 5Kb each - total of 250Kb per sample for 200 samples. We have no lab of our own and would like to run the whole project as a service so we can not rely on home-brewed methods unless they are simple enough to request a service lab to perform. The service lab we have contacted has both MySeq and HiSeq devices and we can probably use other labs with different equipment if preferable.
The biggest part of the cost is the capture kit - we have so far received price quotes for Agilent Haloplex and Ilumina TruSeq, which come out at 350-450$ per sample. As this is over our budget, we are looking for alternative methods which may reduce the cost. MycroArray have a kit named MyBaits which seems to perform the same task at lower cost, but I've not heard feedback from anyone who has used it regarding the quality of the results (% of reads on target etc.).
I'd appreciate any information regarding the MyBaits kit as well as a comparison of cost/ease-of-use/performance of other kits.
We would like to sequence ~50 non-coding areas in the human genome with length of 5Kb each - total of 250Kb per sample for 200 samples. We have no lab of our own and would like to run the whole project as a service so we can not rely on home-brewed methods unless they are simple enough to request a service lab to perform. The service lab we have contacted has both MySeq and HiSeq devices and we can probably use other labs with different equipment if preferable.
The biggest part of the cost is the capture kit - we have so far received price quotes for Agilent Haloplex and Ilumina TruSeq, which come out at 350-450$ per sample. As this is over our budget, we are looking for alternative methods which may reduce the cost. MycroArray have a kit named MyBaits which seems to perform the same task at lower cost, but I've not heard feedback from anyone who has used it regarding the quality of the results (% of reads on target etc.).
I'd appreciate any information regarding the MyBaits kit as well as a comparison of cost/ease-of-use/performance of other kits.
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